一种测定脂质比放射性的单步方法。

A single-step method for determination of the specific radioactivity of lipids.

作者信息

Noble R C, Shand J H

出版信息

Lipids. 1980 Apr;15(4):269-71. doi: 10.1007/BF02535838.

Abstract

The use of a liquid scintillation counter to measure both the mass and the radioactivity content of charred 14C-labeled lipid bands from thin layer chromatoplates has been evaluated. Following lipid mass determination from a measurement of the external standard channels ratio, a suitable choice of counting parameters enabled a reproducible and efficient 14C count to be obtained over virtually the whole range of lipid concentrations tested. Although the charring procedure resulted in some loss of radioactivity, the efficiency of counting remained high enough for accurate dpm measurements.

摘要

已对使用液体闪烁计数器测量来自薄层色谱板的烧焦的14C标记脂质条带的质量和放射性含量进行了评估。通过测量外标通道比确定脂质质量后，选择合适的计数参数能够在几乎整个测试脂质浓度范围内获得可重复且高效的14C计数。尽管炭化过程导致了一些放射性损失，但计数效率仍足够高，可进行准确的每分钟衰变数（dpm）测量。