Yu B P, Bertrand H A, Masoro E J
Metabolism. 1980 May;29(5):438-44. doi: 10.1016/0026-0495(80)90169-9.
Male SPF Fischer 344 rats were fed ad lib. (group A) or 60% ad lib. intake (group R) from 6 wk of age. The group R rats had a markedly increased median length of life. Starting at 6 mo of age, rats were periodically killed and free adipocytes prepared from the epididymal and perirenal depots. The free adipocytes were used for the in vitro study of the effects of age on the promotion of lipolysis by catecholamines. At 6 mo of age, the response of the adipocytes from group A and group R rats was similar when the data are expressed as mumole glycerol released/10(6) adipocytes. With increasing age, however, this lipolytic response to catecholamines of group A rats markedly declined. The catecholamine-stimulated lipolysis/10(6) adipocytes from group R rats changed little between 6 and 18 mo of age, and the decline noted after 18 mo of age was less marked than that occurring in adipocytes from group A rats. The data based on rates of lipolysis/10(6) adipocytes indicate that it is the lifelong nutritional history of a rat population that determines the age-related changes in responsiveness of adipocytes to catecholamines. However, if the data are expressed per sq mm of adipocyte surface, a somewhat different picture emerges. These data are presented and their relevance is discussed.
雄性无特定病原体（SPF）的Fischer 344大鼠从6周龄开始自由进食（A组）或按自由摄入量的60%进食（R组）。R组大鼠的中位寿命显著延长。从6月龄开始，定期处死大鼠，并从附睾和肾周脂肪库制备游离脂肪细胞。这些游离脂肪细胞用于体外研究年龄对儿茶酚胺促进脂肪分解作用的影响。在6月龄时，当数据表示为每10⁶个脂肪细胞释放的甘油微摩尔数时，A组和R组大鼠脂肪细胞的反应相似。然而，随着年龄的增长，A组大鼠对儿茶酚胺的脂肪分解反应明显下降。R组大鼠每10⁶个脂肪细胞的儿茶酚胺刺激的脂肪分解在6至18月龄之间变化不大，18月龄后观察到的下降比A组大鼠脂肪细胞中的下降不明显。基于每10⁶个脂肪细胞脂肪分解率的数据表明，是大鼠群体的终身营养史决定了脂肪细胞对儿茶酚胺反应性的年龄相关变化。然而，如果数据以每平方毫米脂肪细胞表面表示，则会出现略有不同的情况。本文展示了这些数据并讨论了它们的相关性。
