Takagi H, Arakawa A
Res Vet Sci. 1980 Jan;28(1):80-6.
The growth and pathogenicity of Mycoplasma gallisepticum 1RF were studied in chicken tracheal organ cultures. The organism propagated only in the presence of tracheal rings in culture fluids. Presence of metabolic products from rings seems to be an important factor for the growth of organism. Production of hydrogen peroxide was not detected. There were no differences in the growth and cilia-stopping effects among organisms maintained in vitro, stored at -18 degrees C, and recovered from air sac, trachea and joints of infected chickens. No local resistance in tracheal organs from immunised chickens was found. The tracheal organ culture system can readily be used for drug evaluation.
在鸡气管器官培养物中研究了鸡毒支原体1RF的生长和致病性。该生物体仅在培养液中存在气管环的情况下才能繁殖。气管环代谢产物的存在似乎是该生物体生长的一个重要因素。未检测到过氧化氢的产生。在体外培养、-18℃保存以及从感染鸡的气囊、气管和关节中分离出的生物体之间，其生长和纤毛停止效应没有差异。未发现免疫鸡的气管器官存在局部抵抗力。气管器官培养系统可很容易地用于药物评估。
