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用同工酶电泳法对厄瓜多尔并殖吸虫进行虫种鉴定(作者译)

[Species characterization of the lung fluke Paragonimus ecuadoriensis by isoenzyme -electrophoresis (author's transl)].

作者信息

Zillmann U, Voelker J

出版信息

Tropenmed Parasitol. 1980 Mar;31(1):15-20.

PMID:7376248
Abstract

Whole body lysates of 35 adult lung flukes (25 Paragonimus ecuadoriensis, 2 P. africanus, 8 P. uterobilateralis) were electrophorized on thin-layer starch gel. Specific staining was performed for the isoenzymes of the following three enzymes: alanine aminotransferase (ALAT), peptidase 1 (PEP) and malate dehydrogenase (ME) (decarboxylating) (NADP). Two enzymes showed polymorphism and proved to be useful for intra- and interspecific comparison. The individuals of P. ecuadoriensis showed homogeneity and differed remarkably from two other Paragonimus species examined as controls.

摘要

对35条成年肺吸虫(25条厄瓜多尔肺吸虫、2条非洲肺吸虫、8条双侧子宫肺吸虫)的全身裂解物在薄层淀粉凝胶上进行电泳。对以下三种酶的同工酶进行特异性染色:丙氨酸转氨酶(ALAT)、肽酶1(PEP)和苹果酸脱氢酶(脱羧)(NADP)。两种酶表现出多态性,被证明可用于种内和种间比较。厄瓜多尔肺吸虫个体表现出同质性,与作为对照检测的其他两种肺吸虫物种有显著差异。

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