Snape W J, Shiff S, Cohen S
Am J Physiol. 1980 Apr;238(4):G321-5. doi: 10.1152/ajpgi.1980.238.4.G321.
The purpose of the study was to determine the effect of the bile salt, deoxycholic acid, on colonic myoelectrical and contractile activity recorded from a loop of proximal colon in the anesthetized rabbit. Myoelectrical activity was recorded from bipolar electrodes attached to the serosal surface of the proximal colon. Contractile activity of the circular smooth muscle was measured with a strain gauge attached in the circular direction on the serosal surface of the colonic loop. The colonic slow wave frequency was 12.5 +/- 1.1 cycles/min. The slow wave frequency throughout the loop was homogeneous with a low coefficient of variation of the slow wave frequency (5.8 +/- 0.3). There were spike potentials superimposed on 78.1 +/- 3.4% of the slow waves. The addition of 8 or 16 mM deoxycholic acid did not change the slow wave or spike activity. However, deoxycholic acid stimulated a dose-dependent increase in migrating action potential complexes. These complexes were associated with a forceful contraction of the circular smooth muscle that propelled luminal contents in the direction of the burst. Deoxycholic acid also stimulated an increase in luminal fluid content.
本研究的目的是确定胆盐脱氧胆酸对麻醉兔近端结肠肠袢记录的结肠肌电和收缩活动的影响。肌电活动通过连接到近端结肠浆膜表面的双极电极进行记录。环形平滑肌的收缩活动通过沿结肠肠袢浆膜表面圆周方向附着的应变仪进行测量。结肠慢波频率为12.5±1.1次/分钟。整个肠袢的慢波频率均匀，慢波频率的变异系数较低（5.8±0.3）。78.1±3.4%的慢波上叠加有锋电位。添加8或16 mM脱氧胆酸不会改变慢波或锋电位活动。然而，脱氧胆酸刺激迁移性动作电位复合体呈剂量依赖性增加。这些复合体与环形平滑肌的强力收缩相关，该收缩将管腔内内容物沿爆发方向推进。脱氧胆酸还刺激管腔内液体含量增加。
