Barton S P, King C S, Marks R, Nicholls S
Br J Dermatol. 1980 Jan;102(1):63-73. doi: 10.1111/j.1365-2133.1980.tb05673.x.
A technique for the isolation of human stratum corneum cells from any depth within the horny layer is described. Examination of the cells by light, scanning and transmission electron microscope permits a detailed morphological profile of the plasma membrane to be constructed. It is suggested that the technique will be of particular value in determining the effect of chemical agents on the cellular components of the horny layer.
本文描述了一种从角质层内任何深度分离人角质形成细胞的技术。通过光学显微镜、扫描电子显微镜和透射电子显微镜对细胞进行检查,可以构建出质膜的详细形态学特征。建议该技术在确定化学试剂对角质层细胞成分的影响方面具有特殊价值。