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正常和银屑病角质形成细胞的质膜研究。I. 材料制备及形态学特征

Studies on the plasma membrane of normal and psoriatic keratinocytes. I. Preparation of material and morphological characterization.

作者信息

Gommans J M, Bergers M, van Erp P E, van den Hurk J J, Mier P D, Roelfzema H

出版信息

Br J Dermatol. 1979 Oct;101(4):407-12. doi: 10.1111/j.1365-2133.1979.tb00018.x.

DOI:10.1111/j.1365-2133.1979.tb00018.x
PMID:508606
Abstract

A method is described for the preparation of isolated keratinocytes suitable for subsequent biochemical studies. Scanning electron microscopy showed that the maturation process is accompanied by an increase in cell size and a shortening and eventual loss of microvilli. Psoriatic keratinocytes are distinguishable by exhibiting longer microvilli at all levels of maturation.

摘要

本文描述了一种制备适合后续生化研究的分离角质形成细胞的方法。扫描电子显微镜显示,成熟过程伴随着细胞大小的增加以及微绒毛的缩短和最终消失。银屑病角质形成细胞在所有成熟阶段均表现出较长的微绒毛,以此可加以区分。

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Studies on the plasma membrane of normal and psoriatic keratinocytes. I. Preparation of material and morphological characterization.正常和银屑病角质形成细胞的质膜研究。I. 材料制备及形态学特征
Br J Dermatol. 1979 Oct;101(4):407-12. doi: 10.1111/j.1365-2133.1979.tb00018.x.
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Formation of the horny layer in psoriasis--primarily defective or secondarily affected.银屑病中角质层的形成——原发性缺陷还是继发性影响。
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引用本文的文献

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Multiparameter flow cytometric characterization of epidermal cell suspensions prepared from normal and hyperproliferative human skin using an optimized thermolysin-trypsin protocol.使用优化的嗜热菌蛋白酶-胰蛋白酶方案对从正常和增生性人类皮肤制备的表皮细胞悬液进行多参数流式细胞术表征。
Arch Dermatol Res. 1996 Apr;288(4):203-10. doi: 10.1007/BF02505225.
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Cutaneous alkaline phosphatase: a biochemical study.皮肤碱性磷酸酶:一项生化研究。
Arch Dermatol Res. 1982;274(3-4):221-7. doi: 10.1007/BF00403725.
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Changes in suspensions of human keratinocytes due to trypsin.
胰蛋白酶导致的人角质形成细胞悬液的变化。
Arch Dermatol Res. 1981;271(3):245-57. doi: 10.1007/BF00409453.
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The binding of epidermolytic toxin from Staphylococcus aureus to mouse epidermal tissue.金黄色葡萄球菌表皮溶解毒素与小鼠表皮组织的结合。
Histochem J. 1987 Mar;19(3):137-49. doi: 10.1007/BF01695138.
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Dolichos biflorus agglutinin (DBA) reveals a similar basal cell differentiation in normal and psoriatic epidermis.双花扁豆凝集素(DBA)显示正常和银屑病表皮中存在相似的基底细胞分化。
Histochemistry. 1989;93(2):129-32. doi: 10.1007/BF00315965.