Namiki O, Faris B, Tschopp F, Füglistaller P, Hollander W, Franzblau C, Schmid K
Biochemistry. 1980 Apr 29;19(9):1900-4. doi: 10.1021/bi00550a026.
Rabbit aortic smooth muscle cells were evaluated for their ability to synthesize and accumulate glycosaminoglycans (GAGs). Because of the sensitivity of the microtechniques utilized, it is possible to determine the specific radioactivity of the GAGs obtained after radioactive incorporation of [35S]SO4(2-) and [14C]glucosamine. Data obtained at various incubation times indicate that the distribution of the GAGs secreted by the cells into the medium is different from that retained by the cell layer. Hyaluronic acid was shown to be the most abundantly produced GAG, and much of this GAG does not appear to be incorporated into the extracellular matrix. Also, a high percentage of the total chondroitin sulfate B synthesized was secreted into the medium. On the other hand, most of the heparan sulfate and chondroitin sulfate C/A synthesized seems to be associated with the cell layer. These results are consistent with those found in whole rabbit aorta.
对兔主动脉平滑肌细胞合成和积累糖胺聚糖（GAGs）的能力进行了评估。由于所采用的微量技术的敏感性，可以确定在[35S]SO4(2-)和[14C]葡糖胺放射性掺入后获得的GAGs的比放射性。在不同孵育时间获得的数据表明，细胞分泌到培养基中的GAGs的分布与细胞层保留的GAGs的分布不同。透明质酸被证明是产生最多的GAG，并且这种GAG的大部分似乎没有掺入细胞外基质中。此外，合成的硫酸软骨素B总量中有很大一部分分泌到了培养基中。另一方面，合成的硫酸乙酰肝素和硫酸软骨素C/A大部分似乎与细胞层相关。这些结果与在整个兔主动脉中发现的结果一致。
