Ohishi H, Hess D, Kosakai M, Schmid K, Hausmann J, Mogayzel P J, Faris B, Franzblau C
Department of Biochemistry, Boston University School of Medicine, Boston University Medical Center, MA 02118.
Atherosclerosis. 1988 Jan;69(1):61-8. doi: 10.1016/0021-9150(88)90289-4.
In the present study the biosynthesis of glycosaminoglycans (GAGs) by neonatal rat aortic smooth muscle cells in culture was studied. Heparan sulfate (HS) was the predominant GAG of the cell layer accounting for 32-49% of the total GAGs depending on the time in culture. The presence of low sulfated chondroitin sulfate (LSC) in aortic smooth muscle cell cultures is reported here for the first time. The effect of ascorbate on the synthesis and accumulation of these macromolecules resulted in a relative increase of C4S and DS in the cell layer. In contrast, the distribution of the GAGs which were secreted into the medium was not significantly effected by the addition of ascorbate. While HS was always found to be a minor component, the other GAGs were present in about equal concentrations. The total GAG accumulation in the medium was much greater (91-97%) than that of the cell layer (3-9%) indicating that the cells are synthesizing relatively large amounts of GAGs, although incorporation of these macromolecules into the extracellular matrix was consistently low.
在本研究中，对培养的新生大鼠主动脉平滑肌细胞中糖胺聚糖（GAGs）的生物合成进行了研究。硫酸乙酰肝素（HS）是细胞层中主要的GAG，根据培养时间的不同，占总GAGs的32%-49%。本文首次报道了主动脉平滑肌细胞培养物中低硫酸化硫酸软骨素（LSC）的存在。抗坏血酸对这些大分子合成和积累的影响导致细胞层中C4S和DS相对增加。相反，添加抗坏血酸对分泌到培养基中的GAGs分布没有显著影响。虽然HS一直是次要成分，但其他GAGs的浓度大致相等。培养基中GAG的总积累量比细胞层中的积累量（3%-9%）大得多（91%-97%），这表明细胞合成了相对大量的GAGs，尽管这些大分子整合到细胞外基质中的比例一直很低。
