Colard O, Bard D, Bereziat G, Polonovski J
Biochim Biophys Acta. 1980 Apr 18;618(1):88-97. doi: 10.1016/0005-2760(80)90056-9.
Phospholipid acyltransferase activities of plasma membranes have been investigated with various acyl-CoA thioesters (palmitoyl, stearoyl, oleoyl, linoleoyl and arachidonoyl) with and without added lysoderivatives. Different patterns of incorporation were observed for each acyl-CoA into endogenous phosphatidylcholine and phosphatidylethanolamine. The turnover rates calculated with tracer amounts of 10 microM acyl-CoA thioesters were five times faster for the polyunsaturated than for the saturated acyl moieties of phosphatidylethanolamine and phosphatidylcholine. Arachidonoyl-CoA was the best acyl donor at low concentrations and the maximal turnover rate was observed at about 25 microM. No saturation appeared at up to 100 microM linoleoyl-CoA. Linoleoyl-CoA transacylase acylated the lyso-compounds in the following order: lysophosphatidylcholine greater than lysophosphatidylserine and lysophosphatidylinositol, while lysophosphatidylethanolamine inhibited linoleate incorporation into the phosphatidylethanolamine itself. Linoleoyl-CoA transacylation was not affected by the fatty acyl moiety at the 1-position of the lysophosphatidylcholine. The results support the view that the plasma membrane acyltransferase activity might contribute to the formation of bile phosphatidylcholines.
已经使用各种酰基辅酶A硫酯（棕榈酰、硬脂酰、油酰、亚油酰和花生四烯酰）在添加和不添加溶血衍生物的情况下研究了质膜的磷脂酰转移酶活性。观察到每种酰基辅酶A掺入内源性磷脂酰胆碱和磷脂酰乙醇胺的模式不同。用示踪量的10 microM酰基辅酶A硫酯计算的周转率，对于磷脂酰乙醇胺和磷脂酰胆碱的多不饱和酰基部分来说，是饱和酰基部分的五倍。花生四烯酰辅酶A在低浓度下是最佳的酰基供体，最大周转率在约25 microM时观察到。高达100 microM的亚油酰辅酶A时未出现饱和现象。亚油酰辅酶A转酰酶将溶血化合物酰化的顺序如下：溶血磷脂酰胆碱大于溶血磷脂酰丝氨酸和溶血磷脂酰肌醇，而溶血磷脂酰乙醇胺抑制亚油酸掺入磷脂酰乙醇胺本身。溶血磷脂酰胆碱1位的脂肪酰基部分不影响亚油酰辅酶A的转酰作用。结果支持质膜酰基转移酶活性可能有助于胆汁磷脂酰胆碱形成的观点。
