Gussakovskiĭ E E, Abramov A A, Babaev T A, Gulamov T G, Gorina O V
Biokhimiia. 1980 May;45(5):802-5.
The extinction coefficient at 280 nm of the solution (pH 7,2-7,4) of thyroglobulin from normal thyroid and nodular euthyroid goiter was measured with the values of 0,6-0,7 to 0,9 ml.mg-1.cm-1. The value of E1%280 as function of the iodoamino acid content depends on the morphological state of the thyroid gland. A simple method for determination of the E1%280 value by measuring of the protein absorption spectrum is proposed.
对来自正常甲状腺和结节性甲状腺肿的甲状腺球蛋白溶液（pH 7.2 - 7.4）在280 nm处的消光系数进行了测量，其值为0.6 - 0.7至0.9 ml·mg⁻¹·cm⁻¹。作为碘氨基酸含量函数的E1%280值取决于甲状腺的形态状态。提出了一种通过测量蛋白质吸收光谱来测定E1%280值的简单方法。
