Keefer D A, Chung J W, Heimer L
Brain Res. 1980 Jun 9;191(2):301-11. doi: 10.1016/0006-8993(80)91282-2.
'Golgi-like' labeling of anterior thalamic neurons was achieved by injecting horseradish peroxidase dissolved in dimethylsulfoxide (DMSO) into the striatum of the rabbit. Forty-eight hours after injection, elements of the presumed thalamocortical population of anterior thalamic neurons are diffused filled with HRP to the extent that their dendritic arborizations, and even spinous processes, can be readily characterized. Ultrastructurally, these solidly labeled thalamic neurons are seen to contain large, irregular electron dense granules dispersed within a moderately electron dense cytoplasm.
通过将溶解于二甲基亚砜(DMSO)的辣根过氧化物酶注射到兔子的纹状体中,实现了丘脑前核神经元的“高尔基样”标记。注射后48小时,丘脑前核神经元假定的丘脑皮质群体的细胞成分被HRP弥漫性填充,以至于它们的树突分支甚至棘突都能很容易地被识别出来。在超微结构上,可以看到这些被牢固标记的丘脑神经元含有大的、不规则的电子致密颗粒,散布在中等电子密度的细胞质中。