Manso-Martínez R, Villasante A, Avila J
Eur J Biochem. 1980 Apr;105(2):307-13. doi: 10.1111/j.1432-1033.1980.tb04502.x.
Purified high-molecular-weight microtubule-associated protein 2 (MAP2) labelled with [32P]-phosphate has been obtained and used as a marker to study the incorporation of this protein into microtubules at steady state in vitro. The incorporation kinetics of protein MAP2 show two different mechanisms of addition of this protein into microtubules. A fast incorporation, which proceeds essentially independently of microtubule assembly, indicates its addition into binding positions which may be available along the structure. Once the microtubule has been saturated with the protein, its incorporation proceeds from one end of the microtubule, in a polymerization-dependent manner, at a slower rate. Loss of the protein from microtubules at steady state proceeds fundamentally at the opposite end.
已获得用[32P] - 磷酸盐标记的纯化高分子量微管相关蛋白2（MAP2），并将其用作标记物来研究该蛋白在体外稳态下掺入微管的情况。蛋白MAP2的掺入动力学显示出该蛋白掺入微管的两种不同机制。一种快速掺入，其基本独立于微管组装进行，表明它添加到沿结构可能存在的结合位置。一旦微管被该蛋白饱和，其掺入就以聚合依赖性方式从微管的一端开始，速率较慢。在稳态下，蛋白从微管的损失基本上在相反的一端发生。
