Bast B J, Manten-Slingerland R, Roholl P, Meyling F G, Ballieux R E
Eur J Immunol. 1980 Mar;10(3):192-7. doi: 10.1002/eji.1830100307.
The presence of Fc receptors (FcR) on rabbit peripheral blood leukocytes is demonstrated using rosette formation with an ox erythrocyte-antibody (EoxA) complex. The receptor is specific for the Fc fragment of IgG (neither IgM nor F (ab')2 anti-Eox mediates rosette formation) that is antigen-bound (aggregated rabbit IgG inhibits the rosette formation only transiently). The receptor is species-specific: guinea pig IgG/Eox, goat IgG/Eox and sheep IgG/Eox complexes do not show rosette formation, and goat IgG aggregates do not inhibit rosette formation. The origin of the target erythrocytes is of importance. Sheep erythrocytes are not useful, and within Eox large differences between donors were found. Rosette formation was only inhibited by pretreatment of the rosette-forming cells with homologous immune complexes, whereas the size of the antigen greatly influenced the degree of inhibition. The rabbit FcR is pronase-resistant, unlike the human and murine RcR. The interaction of IgG and the FcR is not inhibited by isolated C gamma 3 domains. Further evidence for the requirement of the whole Fc region was obtained in experiments where inhibition of the rosette formation was observed using antisera directed to the C gamma 3 and the C gamma 2 domain, respectively. Anti-Fab antiserum did not inhibit rosette formation. Results are discussed in relation to the mechanism of allotypic suppression.
利用兔外周血白细胞与氧合红细胞 - 抗体（EoxA）复合物形成花环，证明了兔外周血白细胞上存在Fc受体（FcR）。该受体对与抗原结合的IgG的Fc片段具有特异性（IgM和F(ab')2抗Eox均不介导花环形成）（聚集的兔IgG仅短暂抑制花环形成）。该受体具有种属特异性：豚鼠IgG/Eox、山羊IgG/Eox和绵羊IgG/Eox复合物均不显示花环形成，山羊IgG聚集物也不抑制花环形成。靶红细胞的来源很重要。绵羊红细胞无用，并且在Eox中发现不同供体之间存在很大差异。花环形成仅通过用同源免疫复合物预处理花环形成细胞来抑制，而抗原的大小极大地影响抑制程度。与人和鼠的RcR不同，兔FcR对链霉蛋白酶具有抗性。IgG与FcR的相互作用不受分离的Cγ3结构域的抑制。在分别使用针对Cγ3和Cγ2结构域的抗血清观察到花环形成受到抑制的实验中，获得了关于整个Fc区域必要性的进一步证据。抗Fab抗血清不抑制花环形成。结合同种异型抑制机制对结果进行了讨论。
