Zaviacic M, Brozman M, Jakubovský J, Mikulecký M, Blazeková J
Exp Pathol (Jena). 1980;18(2):85-95. doi: 10.1016/s0014-4908(80)80057-3.
The ultrastructure of rat parietal cells of the gastric mucosa was investigated during the twenty-four hours of a day. Male rats were housed at ad libitum feeding under normal light conditions with dark night. The animals were sacrificed at 6.00 h, 12.00 h, 18.00 h and 24.00 h respectively, in groups of 5 animals after standard 24 hours of starvation. From this material the electronograms of 356 parietal cells (19% in 6.00 h, 26% in 12.00 h, 21% in 18.00 h and 34% in 24.00 h samples) were evaluated. Based on literary data, the parietal cells were specified as secreting parietal cells (S, 38% of the total), secreting parietal cells returning to resting state (SR, 18% of the total), resting parietal cells (R, 35% of the total) and resting parietal cells tending to early secreting state (RS, 9% of the total). Some types of parietal cells are statistically highly significantly (x2 = 130.9, p less than 0.001) unequally distributed during the circadian rhythm: S are less numerous at 6.00 h (2% of the total) and 12.00 h (4% of the total) than expected (7 and 10% respectively), and more numerous at 18.00 h (11% of the total) and 24.00 h (21% of the total) than expected (8 and 13% respectively). Conversely, R are more numerous in the morning (13 and 15% instead of 7 and 9% respectively) and less in evening samples (4 and 3% instead of 7 and 12% respectively). Distribution differences were proved statistically (x2-test) for all cell-cell and hour-hour combinations expect the combinations S-RS and 6.00 h to 12.00 h. The maximal differences in distribution were found to be between the amounts of S and R at 6.00 h and 24.00 h (x2 = 77.3, p less than 0.001) and at 12.00 h and 24.00 h (x2 = 69.3, p less than 0.001). Thus, a distinct circadian rhythm of parietal cells, especially as to their fine cell structures involved in acid production was demonstrated. The results render further evidence of the rhythmicity of gastric acid production in rats.
在一天24小时内对大鼠胃黏膜壁细胞的超微结构进行了研究。雄性大鼠在正常光照条件下自由进食，夜间黑暗。在标准饥饿24小时后，分别于6.00、12.00、18.00和24.00时将动物处死，每组5只。从这些材料中评估了356个壁细胞的电子显微照片（6.00时样本中占19%，12.00时样本中占26%，18.00时样本中占21%，24.00时样本中占34%）。根据文献数据，壁细胞分为分泌型壁细胞（S，占总数的38%）、恢复到静止状态的分泌型壁细胞（SR，占总数的18%）、静止壁细胞（R，占总数的35%）和趋于早期分泌状态的静止壁细胞（RS，占总数的9%）。某些类型的壁细胞在昼夜节律中分布不均，具有统计学高度显著性差异（x2 = 130.9，p小于0.001）：S在6.00时（占总数的2%）和12.00时（占总数的4%）比预期数量少（分别应为7%和10%），而在18.00时（占总数的11%）和24.00时（占总数的21%）比预期数量多（分别应为8%和13%）。相反，R在早晨数量较多（分别为13%和15%，而不是7%和9%），在傍晚样本中数量较少（分别为4%和3%，而不是7%和12%）。除了S-RS组合以及6.00时到12.00时的组合外，所有细胞类型与时间组合的分布差异均经统计学（x2检验）证实。分布差异最大的是6.00时和24.00时以及12.00时和24.00时S和R的数量（x2 = 77.3，p小于0.001；x2 = 69.3，p小于0.001）。因此，证明了壁细胞存在明显的昼夜节律，尤其是与其参与胃酸分泌的精细细胞结构有关。这些结果进一步证明了大鼠胃酸分泌的节律性。
