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成年大鼠心脏单个心肌细胞的电压钳法

Voltage clamp method on single cardiac cells from adult rat heart.

作者信息

Undrovinas A I, Yushmanova A V, Hering S, Rosenshtraukh L V

出版信息

Experientia. 1980 May 15;36(5):572-4. doi: 10.1007/BF01965808.

DOI:10.1007/BF01965808
PMID:7379958
Abstract

Voltage clamp experiments on isolated cardiac cells from adult rat hearts were carried out using an intracellular dialysis method. The fast inward current was recorded. Tetrodotoxin (TTX) at a concentration of 2.5 x 10(-7) g/ml blocked this current to 30% of its initial value. Inward maximal fast current density was calculated to be 0.14--0.7 mA/cm2.

摘要

采用细胞内透析法对成年大鼠心脏分离的心肌细胞进行电压钳实验。记录快速内向电流。浓度为2.5×10⁻⁷g/ml的河豚毒素(TTX)将该电流阻断至其初始值的30%。计算得出内向最大快速电流密度为0.14 - 0.7 mA/cm²。

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引用本文的文献

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本文引用的文献

1
A quantitative description of membrane current and its application to conduction and excitation in nerve.膜电流的定量描述及其在神经传导和兴奋中的应用。
J Physiol. 1952 Aug;117(4):500-44. doi: 10.1113/jphysiol.1952.sp004764.
2
Heart: excitation and contraction.心脏:兴奋与收缩。
Annu Rev Physiol. 1971;33:479-532. doi: 10.1146/annurev.ph.33.030171.002403.
3
Voltage clamping of multicellular myocardial preparations: capabilities and limitations of existing methods.多细胞心肌制剂的电压钳制:现有方法的能力与局限
J Gen Physiol. 1981 Jul;78(1):19-42. doi: 10.1085/jgp.78.1.19.
4
Effect of extracellular pH on sodium current in isolated, single rat ventricular cells.细胞外pH值对离体单个大鼠心室肌细胞钠电流的影响。
J Membr Biol. 1984;78(2):163-8. doi: 10.1007/BF01869203.
5
Patch-voltage-clamp method for measuring fast inward current in single rat heart muscle cells.用于测量单个大鼠心肌细胞快速内向电流的膜片钳电压钳方法。
Pflugers Arch. 1982 Aug;394(2):150-5. doi: 10.1007/BF00582917.
6
Effects of a new antiarrhythmic compound SUN 1165 [N-(2,6-dimethylphenyl)-8-pyrrolizidineacetamide hydrochloride] on the sodium currents in isolated single rat ventricular cells.一种新型抗心律失常化合物SUN 1165 [N-(2,6-二甲基苯基)-8-吡咯烷乙酰胺盐酸盐] 对离体单个大鼠心室细胞钠电流的影响。
Naunyn Schmiedebergs Arch Pharmacol. 1984 Jun;326(2):163-8. doi: 10.1007/BF00517314.
7
A comparative electrophysiological study of enzymatically isolated single cells and strips of frog ventricle.蛙心室酶解分离单细胞与肌条的比较电生理研究。
Pflugers Arch. 1985 Oct;405(3):274-84. doi: 10.1007/BF00582572.
8
Sodium current in single myocardial mouse cells.单个小鼠心肌细胞中的钠电流。
Pflugers Arch. 1985 May;404(2):190-6. doi: 10.1007/BF00585418.
Prog Biophys Mol Biol. 1978;34(3):219-54. doi: 10.1016/0079-6107(79)90019-1.
4
A rapid method for the isolation of viable cardiac myocytes from adult rat.一种从成年大鼠中分离有活力心肌细胞的快速方法。
Exp Cell Res. 1978 Aug;115(1):183-9. doi: 10.1016/0014-4827(78)90415-9.
5
Surface characteristics of cells isolated from adult rat myocardium.从成年大鼠心肌中分离出的细胞的表面特征。
J Mol Cell Cardiol. 1978 Mar;10(3):287-92. doi: 10.1016/0022-2828(78)90351-6.
6
Blockade of Ca2+ dependent rat atrial slow action potentials by adenosine and lanthanum.
Pflugers Arch. 1979 May 15;380(1):19-27. doi: 10.1007/BF00582607.
7
Ionic currents in the neuroblastoma cell membrane.神经母细胞瘤细胞膜中的离子电流。
Neuroscience. 1978;3(3):327-32. doi: 10.1016/0306-4522(78)90080-5.
8
Sodium current in single heart muscle cells.单个心肌细胞中的钠电流。
Nature. 1979 Mar 15;278(5701):269-71. doi: 10.1038/278269a0.
9
Sodium channels in rabbit cardiac Purkinje fibres.兔心脏浦肯野纤维中的钠通道。
Nature. 1979 Mar 15;278(5701):265-8. doi: 10.1038/278265a0.