Kunishi K
Gastroenterol Jpn. 1980 Apr;15(2):144-7. doi: 10.1007/BF02774928.
Secretory component (SC) isolated from human colostrum was radioiodinated with chloramin-T method and it was subjected to a radioimmunoassay for quantitation of SC. A monospecific rabbit anti-sera against human SC which could react with free SC as well as immunoglobulin bound SC was used for the assay. The present method can measure as low as 300 ng/ml of SC. The level (mean +/- 2 S.D.) of serum SC in healthy subjects was determined to be 31.3 +/- 7.2 micrograms/ml. Studies with Sephadex G-200 gel filtration demonstrated that SC in the normal sera existed mostly as a form of SC-IgA. In contrast, free SC was never detected in them.
从人初乳中分离出的分泌成分（SC）用氯胺 - T法进行放射性碘化，并进行放射免疫测定以定量SC。使用针对人SC的单特异性兔抗血清进行测定，该抗血清可与游离SC以及免疫球蛋白结合的SC发生反应。本方法可检测低至300 ng/ml的SC。健康受试者血清SC水平（平均值±2标准差）测定为31.3±7.2微克/毫升。用葡聚糖凝胶G - 200凝胶过滤研究表明，正常血清中的SC大多以SC - IgA的形式存在。相反，在这些血清中从未检测到游离SC。
