Kanazawa K, Mitsuoka T, Arai K, Yamamoto T, Hino Y
Gastroenterol Jpn. 1980 Apr;15(2):177-83. doi: 10.1007/BF02774934.
Quantitative analysis of intestinal microflorae in the diverted and feces-containing portions of the colon after performing an ascendo-descendostomy Roux en Y in Wistar-Lewis rats, the colonic portion from the ascending colon to the midportion of the descending colon being diverted from the fecal stream, disclosed a drastic decrease in the total amount of the intestinal microflora as well as most anaerobic microflorae in the diverted portion of the colon where the amount of the colonic content was extremely reduced. This diverted segment was least susceptible of developing macroscopical epithelial neoplasia after exposure to a carcinogen, MNNG, sufficient in amount to evoke multiple large neoplasia in the ordinary colon, although microscopical intramucosal neoplastic foci were induced there. Thus the existence of the intestinal content was essential for the process of promotion in experimental colonic carcinogenesis. The close parallelism between amounts of intestinal content and amounts of intestinal microflorae, especially anaerobic ones, suggested the roles of anaerobic microflorae in colonic carcinogenesis.
对Wistar-Lewis大鼠施行顺行-逆行吻合Roux-en-Y术式后，对结肠转流段和含粪便段的肠道菌群进行定量分析，结果显示，从升结肠至降结肠中部的结肠段转流至粪流之外，该转流段结肠内容物量极少，肠道菌群总量以及大多数厌氧菌群数量急剧减少。尽管该转流段在显微镜下可诱导黏膜内肿瘤病灶，但在接触足以在正常结肠诱发多个大肿瘤的致癌物N-甲基-N'-硝基-N-亚硝基胍（MNNG）后，该转流段发生肉眼可见上皮肿瘤的可能性最小。因此，肠道内容物的存在对于实验性结肠癌发生的促进过程至关重要。肠道内容物量与肠道菌群量（尤其是厌氧菌群）之间密切的平行关系提示了厌氧菌群在结肠癌发生中的作用。
