Harty R F, McGuigan J E
Gastroenterology. 1980 May;78(5 Pt 1):925-30.
The purpose of the present study was to examine the effects of the cholinergic agent carbachol on gastrin synthesis and secretion by rat antral mucosa in organ culture. In addition, the effect of atropine on basal and carbachol-stimulated gastrin release was investigated. These in vitro studies demonstrate that carbachol stimulated both gastrin secretion and synthesis in a dose-dependent manner. Maximal stimulation of gastrin synthesis and release occurred at a concentration of 1 X 10(-5) M carbachol. The rate of gastrin release stimulated by carbachol (1 X10(-5) M) was 0.79 ng-hr-1-mg-1 which was significantly greater than the control value of 0.37 ng-hr-1 (P less than 0.001). Atropine, over the dose range from 10(-7) to 10(-3) M, had no effect on basal (unstimulated) gastrin release. However, carbachol-stimulated gastrin release was inhibited progressively by inclusion of increasing concentrations of atropine in the culture media. The results of these studies indicated that the acetylcholine analogue, carbachol, is capable of directly stimulating the antral gastrin cell to significantly increase the rate of synthesis and release of gastrin. Whereas atropine, under these in vitro conditions, does not alter basal gastrin release, the muscarinic antagonist does competively inhibit carbachol-stimulated gastrin secretion.
本研究的目的是检测胆碱能药物卡巴胆碱对器官培养的大鼠胃窦黏膜胃泌素合成与分泌的影响。此外，还研究了阿托品对基础状态及卡巴胆碱刺激的胃泌素释放的影响。这些体外研究表明，卡巴胆碱以剂量依赖方式刺激胃泌素的分泌与合成。胃泌素合成与释放的最大刺激发生在卡巴胆碱浓度为1×10⁻⁵ M时。卡巴胆碱（1×10⁻⁵ M）刺激的胃泌素释放速率为0.79 ng·hr⁻¹·mg⁻¹，显著高于对照值0.37 ng·hr⁻¹（P<0.001）。在10⁻⁷至10⁻³ M的剂量范围内，阿托品对基础（未刺激）胃泌素释放无影响。然而，在培养基中加入浓度不断增加的阿托品会逐渐抑制卡巴胆碱刺激的胃泌素释放。这些研究结果表明，乙酰胆碱类似物卡巴胆碱能够直接刺激胃窦胃泌素细胞，显著提高胃泌素的合成与释放速率。而在这些体外条件下，阿托品不改变基础胃泌素释放，但毒蕈碱拮抗剂确实竞争性抑制卡巴胆碱刺激的胃泌素分泌。
