[Basic peptides in been venom, V. Synthesis of four fragments from the sequence of apamin (author's transl)].

The synthesis of the following four fragments by conventional methods is described: Pos. 15-17 Boc-Cys(SiPr)-Gln(Mbh)-Gln(Mbh) (I) Pos. 10-14 Boc-Leu-Cys(Trt)-Ala-Arg(Tos)-Arg(Tos) (II) Pos. 5-9 Boc-Ala-Pro-Glu(gammaBzl)-Thr-Ala (III) Pos. 1-4 Boc-Cys(SiPr)-Asn-Cys(SiPr)-Lys(Z) (IV) These peptides are fragments of apamin, a basic, neurotoxic peptide from bee venom. The purity of the products was examined by thin-layer chromatography, amino acid and elemental analysis. It is possible to synthesize apamin by liquid-phase fragment condensation coupling these peptides stepwise on a polyethylene-histidine support.