Gupta M N, Murthy G S, Vithayathil P J
Int J Pept Protein Res. 1980 Mar;15(3):243-7. doi: 10.1111/j.1399-3011.1980.tb02573.x.
OBQ reacts specifically with methionine residues of RNAase A at highly acidic pH. At pH 3, whereas RNAase A does not react, RNAase S, S-protein and S-peptide all react with OBQ. RNAase A derivatives in which some of the methionines have been initially modified at pH 1, further react with OBQ at pH 3. Thus, reaction with OBQ can be used to monitor structural transitions and conformational differences involving methionines in proteins.
OBQ在高酸性pH值下与核糖核酸酶A的甲硫氨酸残基发生特异性反应。在pH 3时，核糖核酸酶A不发生反应，而核糖核酸酶S、S蛋白和S肽均与OBQ发生反应。其中一些甲硫氨酸在pH 1时已预先修饰的核糖核酸酶A衍生物，在pH 3时进一步与OBQ发生反应。因此，与OBQ的反应可用于监测涉及蛋白质中甲硫氨酸的结构转变和构象差异。
