van Egmond H P, Paulsch W E, Deijll E, Schuller P L
J Assoc Off Anal Chem. 1980 Jan;63(1):110-4.
A semi-quantitative method is described for the analysis of sterigmatocystin in cheese. The method is based on extraction of cheese with MeOH--4% KCl (9 + 1), followed by Florisil and polyamide column cleanup and 2-dimensional thin layer chromatography (TLC). Visualization of sterigmatocystin on the TLC plates is enhanced by an AlCl3 spray reagent. The identity of sterigmatocystin is confirmed by a 2-dimensional TLC test based on reaction with trifluoroacetic acid (TFA) on the plate after first development. The reaction product formed is visualized by spraying with AlCl3. The method allows detection and confirmation of sterigmatocystin in cheese at concentrations as low as 5 micrograms/kg. The method has been applied to cheese samples ripening in warehouses and naturally molded with Aspergillus versicolor.
描述了一种用于分析奶酪中柄曲霉素的半定量方法。该方法基于用甲醇 - 4%氯化钾（9 + 1）提取奶酪，随后进行弗罗里硅土和聚酰胺柱净化以及二维薄层色谱（TLC）。通过氯化铝喷雾试剂可增强柄曲霉素在TLC板上的显色效果。通过在首次展开后基于板上与三氟乙酸（TFA）反应的二维TLC试验来确认柄曲霉素的身份。形成的反应产物通过喷氯化铝来显色。该方法能够检测和确认奶酪中低至5微克/千克浓度的柄曲霉素。该方法已应用于在仓库中成熟并自然被杂色曲霉污染的奶酪样品。
