尾草履虫行为突变体在接合过程中及通过显微注射野生型细胞质恢复膜兴奋性。
Restoration of membrane excitability in a behavioral mutant of Paramecium caudatum during conjugation and by microinjection of wild-type cytoplasm.
作者信息
Hiwatashi K, Haga N, Takahashi M
出版信息
J Cell Biol. 1980 Feb;84(2):476-80. doi: 10.1083/jcb.84.2.476.
Abstract
When cells of the behavioral mutant cnrC of Paramecium caudatum were mated with the wild type, phenotype change from CNR (no backward swinning) to wild type in the cnrC mate occurred immediately after the formation of tight pairs. No change of phenotype occurred when cells of cnrA or cnrB were mated with wild type. Phenotypic change from CNR to wild type in cells of cnrC was also induced by microinjection of wild-type cytoplasm. Microinjection of wild-type cytoplasm induced no change in cells of cnrA or cnrB. Phenotypic change in the cnrC mate during conjugation can be explained by cytoplasmic exchange during conjugation, though transfer of membrane sites for excitability through membrane fluidity cannot be ruled out.
摘要
当尾草履虫行为突变体cnrC的细胞与野生型细胞交配时，cnrC交配型中从CNR（无向后游动）到野生型的表型变化在紧密配对形成后立即发生。当cnrA或cnrB的细胞与野生型交配时，未发生表型变化。通过显微注射野生型细胞质也可诱导cnrC细胞中从CNR到野生型的表型变化。显微注射野生型细胞质未在cnrA或cnrB细胞中诱导变化。接合期间cnrC交配型中的表型变化可以通过接合期间的细胞质交换来解释，尽管通过膜流动性转移兴奋性膜位点也不能排除。
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