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气相色谱法测定健康儿童红细胞膜缩醛磷脂的脂肪酸模式

Gas chromatographic determination of the fatty acid pattern of red cell membrane plasmalogens in healthy children.

作者信息

Rogiers V

出版信息

J Chromatogr. 1980 Apr 11;182(1):27-33. doi: 10.1016/s0378-4347(00)81647-3.

DOI:10.1016/s0378-4347(00)81647-3
PMID:7380900
Abstract

A gas chromatographic technique for the determination of the fatty acid pattern of the phosphatidylcholine (PC) and phosphatidyl ethanolamine (PE) plasmalogen and non-plasmalogen fractions in human red cell membranes has been developed. The coefficient of variation lies between 5 and 10%. The technique has been applied to blood samples of healthy Belgian school children (8--10 year old). PE contains 47% plasmalogen and 53% non-plasmalogen form, whereas PC occurs only in its non-plasmalogen form. The fatty acid pattern of the various fractions and subfractions has been determined.

摘要

已开发出一种气相色谱技术,用于测定人红细胞膜中磷脂酰胆碱(PC)、磷脂酰乙醇胺(PE)缩醛磷脂和非缩醛磷脂组分的脂肪酸模式。变异系数在5%至10%之间。该技术已应用于比利时健康学童(8至10岁)的血液样本。PE含有47%的缩醛磷脂和53%的非缩醛磷脂形式,而PC仅以其非缩醛磷脂形式存在。已测定了各种组分和亚组分的脂肪酸模式。

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引用本文的文献

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Abnormal fatty acid pattern of the plasma cholesterol ester fraction in cystic fibrosis patients with and without pancreatic insufficiency.患有和未患有胰腺功能不全的囊性纤维化患者血浆胆固醇酯部分的异常脂肪酸模式。
Eur J Pediatr. 1983 Oct;141(1):39-42. doi: 10.1007/BF00445666.
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Molecular species of glycerophospholipids and sphingomyelins of human erythrocytes: improved method of analysis.人红细胞甘油磷脂和鞘磷脂的分子种类:改进的分析方法
Lipids. 1989 May;24(5):396-407. doi: 10.1007/BF02535147.
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Age-dependent fatty acid composition of erythrocyte membrane phospholipids in healthy children.
健康儿童红细胞膜磷脂的年龄依赖性脂肪酸组成
Z Ernahrungswiss. 1990 Dec;29(4):284-94. doi: 10.1007/BF02023085.