血液、血浆和尿液中多沙普仑的定量分析。

Quantitation of doxapram in blood, plasma and urine.

作者信息

Nichol H, Vine J, Thomas J, Moore R G

出版信息

J Chromatogr. 1980 May 9;182(2):191-200. doi: 10.1016/s0378-4347(00)81623-0.

Abstract

Methods for the quantitation of doxapram in blood, plasma and urine have been developed. Following extraction, gas-liquid chromatography was used to separate doxapram from basic metabolites. Doxapram was detected by mass spectrometry for blood and plasma assays, and by flame ionisation for urine assays. The limit of reliable quantitation in blood and plasma was 10 ng and in urine 500 ng, the coefficients of variation being 6.37%, 1.72% and 2.31% respectively. To illustrate the clinical applicability of the assay methods, plasma, blood and urine levels were monitored in a premature newborn following an intravenous infusion of doxapram.

摘要

已开发出血液、血浆和尿液中多沙普仑的定量方法。提取后，采用气液色谱法将多沙普仑与碱性代谢物分离。血液和血浆分析采用质谱法检测多沙普仑，尿液分析采用火焰离子化法检测。血液和血浆中可靠定量的下限为10纳克，尿液中为500纳克，变异系数分别为6.37%、1.72%和2.31%。为说明该检测方法的临床适用性，对一名早产新生儿静脉输注多沙普仑后监测其血浆、血液和尿液水平。