通过固相酶联免疫吸附测定法检测人血清中特异性抗钩端螺旋体免疫球蛋白M和G
Detection of specific anti-leptospiral immunoglobulins M and G in human serum by solid-phase enzyme-linked immunosorbent assay.
作者信息
Adler B, Murphy A M, Locarnini S A, Faine S
出版信息
J Clin Microbiol. 1980 May;11(5):452-7. doi: 10.1128/jcm.11.5.452-457.1980.
Abstract
The enzyme-linked immunosorbent assay (ELISA) was used to detect leptospire-specific immunoglobulin M (IgM) and IgG in the sera of patients infected with leptospiral serovars hardjo, pomona, or copenhageni. All patients produced specific IgM and IgG detectable by ELISA. In contrast, only a few patients produced IgG agglutinins whereas all produced IgM agglutinins. The specificity and sensitivity of the test suggest that the ELISA anti-IgM technique is a suitable method for detecting leptospiral antibodies in human sera for diagnostic and epidemiological purposes.
摘要
采用酶联免疫吸附测定法（ELISA）检测感染了哈焦、波摩那或哥本哈根等钩端螺旋体血清型的患者血清中的钩端螺旋体特异性免疫球蛋白M（IgM）和免疫球蛋白G（IgG）。所有患者通过ELISA均可检测到特异性IgM和IgG。相比之下，只有少数患者产生IgG凝集素，而所有患者均产生IgM凝集素。该检测方法的特异性和敏感性表明，ELISA抗 IgM技术是一种适用于检测人血清中钩端螺旋体抗体的诊断和流行病学方法。
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