Guidry A J, Paape M J, Pearson R E
J Dairy Sci. 1980 Apr;63(4):611-5. doi: 10.3168/jds.S0022-0302(80)82979-1.
Milk samples were collected from two quarters in each of 8 cows that had Wisconsin Mastitis Test scores of less than 8.0. After letdown was stimulated, 35 ml of foremilk, the sixth 35 ml (middle milk), and 45 ml of strippings were collected by hand. Measured on each fraction were immunoglobulins, serum albumin, total protein, pH, percentage fat, Wisconsin Mastitis Test, and the ability of skim milk and whole milk to support phagocytosis of Staphylococcus aureus by isolated donor milk neutrophils. Foremilk and middle milk differed in immunoglobulin M (.014 to .018 mg/ml) and in the ability of whole milk to support phagocytosis (32 to 28% S. aureus phagocytosed). The following measures differed from middle milk to strippings: immunoglobulin A (.063 to .083 mg/ml); immunoglobulin G1 (.238 to .302 mg/ml); immunoglobulin M (.018 to .023 mg/ml); fat (3.9 to 7.4%); and Wisconsin Mastitis Test (5.0 to 9.2). Immunoglobulin G2, bovine serum albumin, total protein, pH, and the ability of skim milk and whole milk to support phagocytosis did not change. Although small differences may exist between foremilk and middle milk, foremilk gave reliable estimates.
从8头威斯康星乳房炎检测得分低于8.0的奶牛中，每头采集两个乳腺的乳汁样本。在刺激排乳后，手工采集35毫升的前乳、第六个35毫升（中间乳）和45毫升的末乳。对每个部分检测免疫球蛋白、血清白蛋白、总蛋白、pH值、脂肪百分比、威斯康星乳房炎检测，以及分离出的供体乳中性粒细胞的脱脂乳和全脂乳支持金黄色葡萄球菌吞噬作用的能力。前乳和中间乳在免疫球蛋白M（0.014至0.018毫克/毫升）以及全脂乳支持吞噬作用的能力（32%至28%的金黄色葡萄球菌被吞噬）方面存在差异。从中间乳到末乳，以下指标存在差异：免疫球蛋白A（0.063至0.083毫克/毫升）；免疫球蛋白G1（0.238至0.302毫克/毫升）；免疫球蛋白M（0.018至0.023毫克/毫升）；脂肪（3.9%至7.4%）；以及威斯康星乳房炎检测（5.0至9.2）。免疫球蛋白G2、牛血清白蛋白、总蛋白、pH值，以及脱脂乳和全脂乳支持吞噬作用的能力没有变化。尽管前乳和中间乳之间可能存在细微差异，但前乳能给出可靠的估计值。
