Crowley J P, Valeri C R
J Lab Clin Med. 1980 Jun;95(6):868-76.
The inhibition of killing of Staphylococcus aureus 502A by PMNs treated with the IgG fraction of serum from a group of patients with demonstrable leukocyte antibodies was investigated. The uptake of opsonized thymidine-labeled S. aureus 502A by PMNs treated with allogeneic antibody was essentially unimpaired, despite significantly decreased killing. The findings were similar to bacteria opsonized by serum complement or bacteria opsonized with specific lapine antibody. An increased proportion of PMN-bound bacteria susceptible to lysis by lysostaphin indicated a reduced rate of translocation of bacteria from the surface of allogeneic antibody-treated PMNs. Antibody did not stimulate the basal oxidative metabolism, but the oxidative metabolism of antibody-treated PMNs during phagocytosis was increased. Although the precise mechanism of inhibition of PMN killing by antibody is uncertain, the data suggest that the impairment of bacterial killing by PMNs treated with allogeneic leukocyte antibody is associated with inefficient translocation of bacteria into phagolysosomes rather than by interference with the binding of bacteria to specific PMN opsonic receptors.
对一组有明显白细胞抗体的患者血清IgG组分处理的中性粒细胞（PMN）抑制金黄色葡萄球菌502A杀伤作用进行了研究。尽管杀伤作用显著降低，但用同种异体抗体处理的PMN对经调理的胸腺嘧啶标记的金黄色葡萄球菌502A的摄取基本未受损害。这些发现与经血清补体调理的细菌或用特异性兔抗体调理的细菌相似。对溶葡萄球菌素敏感的结合在PMN上的细菌比例增加，表明同种异体抗体处理的PMN表面细菌转位速率降低。抗体未刺激基础氧化代谢，但吞噬过程中抗体处理的PMN的氧化代谢增加。虽然抗体抑制PMN杀伤的确切机制尚不确定，但数据表明，用同种异体白细胞抗体处理的PMN对细菌杀伤的损害与细菌向吞噬溶酶体的转运效率低下有关，而不是通过干扰细菌与特定PMN调理受体的结合。
