Wilson W G, Kirkman H N, Clemons E H
J Lab Clin Med. 1980 Jun;95(6):888-96.
Resealed red cell ghosts were prepared at a final dilution of 1:325 from normal and G6PD-deficient cells. The activity of the HMS and concentrations of G6P, ATP, and total and reduced nictinamide adenine dinucleotide phosphate were determined after incubation in the presence of 0 and 100 micrometer methylene blue. The results indicate that the HMS is still under ununexplained restraint in the resealed cells but that the restraint has been reduced between twofold and fourfold from that observed in the intact normal red cells. The addition of various combinations of ATP, hexokinase, and methylene blue led to the demonstration that the unexplained restraint is not significantly correlated with levels of ATP or G6P. As with intact red cells, however, the explained restraint is greatest under conditions that cause the level of NADP+ to be low and that of NADPH to be high.
将正常细胞和葡萄糖-6-磷酸脱氢酶（G6PD）缺乏的细胞以1:325的最终稀释度制备重新密封的红细胞血影。在存在0和100微摩尔亚甲蓝的情况下孵育后，测定己糖磷酸支路（HMS）的活性以及葡萄糖-6-磷酸（G6P）、三磷酸腺苷（ATP）和总烟酰胺腺嘌呤二核苷酸磷酸及还原型烟酰胺腺嘌呤二核苷酸磷酸的浓度。结果表明，在重新密封的细胞中，HMS仍受到无法解释的抑制，但这种抑制比在完整的正常红细胞中观察到的减少了两倍至四倍。添加ATP、己糖激酶和亚甲蓝的各种组合表明，这种无法解释的抑制与ATP或G6P的水平没有显著相关性。然而，与完整红细胞一样，在导致烟酰胺腺嘌呤二核苷酸磷酸（NADP+）水平低而还原型烟酰胺腺嘌呤二核苷酸磷酸（NADPH）水平高的条件下，可解释的抑制作用最大。
