Baraud J, Maurice A
J Lipid Res. 1980 Mar;21(3):347-53.
Benzo(a)pyrene injection increased the phospholipid content in membranes of rat liver mitochondria and microsomes. There was a large relative increase of phosphatidylcholine, especially in microsomes, as compared with normal liver. The quantity of phosphatidylethanolamine seemed not be affected and the other phospholipid classes decreased. In vivo [U-14C]glycerol incorporation into phospholipids was greater after benzo(a)pyrene injection, especially into phosphatidylcholine. Liver microsomes derived from rats injected with [U-14C]glycerol or from liver slices incubated with the same precursor were incubated with unlabeled mitochondria in the presence of 105,000 g supernatant fraction; labeled microsomal phospholipids exchanged to a greater extent in the presence of benzo(a)pyrene, suggesting either a stimulation of activity of the exchange proteins, or a relative membrane disorganization facilitating the phospholipid transfer.
注射苯并(a)芘可增加大鼠肝线粒体和微粒体膜中的磷脂含量。与正常肝脏相比，磷脂酰胆碱有较大幅度的相对增加，尤其是在微粒体中。磷脂酰乙醇胺的量似乎未受影响，而其他磷脂种类则减少。注射苯并(a)芘后，体内[U-14C]甘油掺入磷脂的量增加，尤其是掺入磷脂酰胆碱。将注射了[U-14C]甘油的大鼠的肝微粒体或与相同前体一起孵育的肝切片来源的肝微粒体，在105,000g上清液存在的情况下与未标记的线粒体一起孵育；在苯并(a)芘存在的情况下，标记的微粒体磷脂交换程度更大，这表明要么是交换蛋白的活性受到刺激，要么是相对的膜紊乱促进了磷脂转移。
