DNA聚合酶γ在体外催化腺病毒DNA合成。

Catalysis of adenovirus DNA synthesis in vitro by DNA polymerase gamma.

作者信息

Shaw C H, Rekosh D M, Russell W C

出版信息

J Gen Virol. 1980 May;48(1):231-6. doi: 10.1099/0022-1317-48-1-231.

Abstract

DNA replication complexes were purified from adenovirus type 5 (Ad5)-infected HeLa cells. DNA synthesis by these complexes in vitro was extremely sensitive to the inhibitors dideoxythymidine triphosphate, N-ethyl maleimide and p-hydroxymercuribenzoate. The bound DNA polymerase was released from the complexes by limited digestion with micrococcal nuclease. This released polymerase preferred poly(rA):(dT)12-18 as template over activated calf thymus DNA. These results are compatible with the major polymerase in the replication complex being of the gamma class.

摘要

从感染5型腺病毒（Ad5）的HeLa细胞中纯化出DNA复制复合物。这些复合物在体外进行的DNA合成对双脱氧胸苷三磷酸、N-乙基马来酰亚胺和对羟基汞苯甲酸等抑制剂极为敏感。通过用微球菌核酸酶进行有限消化，可从复合物中释放出结合的DNA聚合酶。这种释放出的聚合酶更倾向于以聚（rA）:（dT）12 - 18作为模板，而非活化的小牛胸腺DNA。这些结果与复制复合物中的主要聚合酶属于γ类相符合。