与2型腺病毒复制复合体相关的DNA聚合酶：2'-3'-双脱氧胸苷-5'-三磷酸对病毒DNA合成的影响
The DNA polymerases associated with the adenovirus type 2 replication complex: effect of 2'-3'-dideoxythymidine-5'-triphosphate on viral DNA synthesis.
作者信息
Abboud M M, Horwitz M S
出版信息
Nucleic Acids Res. 1979 Mar;6(3):1025-39. doi: 10.1093/nar/6.3.1025.
Abstract
An adenovirus (Ad) DNA replication complex extracted from infected HeLa nuclei could be purified free of the bulk of intracellular DNA polymerase activity by sedimetation in neutral sucrose gradients. However, the replication complex still retained some alpha and gamma DNA-polymerase activity. Since this complex is inhibited by 2', 3' dideoxythymidine-5'-triphosphate (ddTTP), an inhibitor of DNA polymerase gamma, a functional role for this enzyme in Ad DNA replication is suggested. Similar inhibition by ddTTP in intact Ad infected nuclei and comparable inhibition of Ad DNA synthesis in whole cells by dideoxythymidine (ddThy) are consistent with a role for DNA polymerase gamma. Uninfected HeLa nuclei or whole cells are not similarly inhibited by ddTTP or DDThy respectively. Such data does not rule out an additional functional role for other DNA polymerases, and recent experiments from this laboratory (1) suggest that DNA polymerase alpha is also involved in Ad DNA synthesis.
摘要
从感染的HeLa细胞核中提取的腺病毒（Ad）DNA复制复合物，通过在中性蔗糖梯度中沉降，可被纯化，去除大部分细胞内DNA聚合酶活性。然而，该复制复合物仍保留一些α和γ DNA聚合酶活性。由于该复合物受到DNA聚合酶γ的抑制剂2'，3' - 双脱氧胸苷-5'-三磷酸（ddTTP）的抑制，提示该酶在Ad DNA复制中具有功能性作用。ddTTP在完整的Ad感染细胞核中的类似抑制作用，以及双脱氧胸苷（ddThy）对全细胞中Ad DNA合成的类似抑制作用，都与DNA聚合酶γ的作用一致。未感染的HeLa细胞核或全细胞分别不受ddTTP或ddThy的类似抑制。这些数据并不排除其他DNA聚合酶具有额外的功能性作用，并且本实验室最近的实验（1）表明DNA聚合酶α也参与Ad DNA合成。
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