鸡胚核苷磷酸转移酶的部分纯化及某些性质

Partial purification and some properties of a nucleoside phosphotransferase of chick embryos.

作者信息

Tesoriere G, Vento R, Calvaruso G, Taibi G

出版信息

Experientia. 1978 Oct 15;34(10):1268-70. doi: 10.1007/BF01981409.

DOI:10.1007/BF01981409

Abstract

A nucleoside phosphotransferase purified about 40fold from chick embryos utilizes efficiently as phosphate donors deoxyribonucleoside and pyrimidine ribonucleoside monophosphates, whereas the pyrimidine deoxyribonucleoside appear to be the preferred acceptors of phosphate. The enzyme is very unstable to heat, dilution and dialysis. A marked enhancement in the stability is caused by nucleotides and it seems associated with the formation of an aggregated state of the protein.

摘要

从鸡胚中纯化得到的一种核苷磷酸转移酶，其活性提高了约40倍，它能有效地利用脱氧核糖核苷单磷酸和嘧啶核糖核苷单磷酸作为磷酸供体，而嘧啶脱氧核糖核苷似乎是磷酸的首选受体。该酶对热、稀释和透析非常不稳定。核苷酸可显著提高其稳定性，这似乎与蛋白质聚集态的形成有关。