一种使用垂直管转头进行等密度离心来快速分离和分级蛋白聚糖的方法。
A rapid method of isolation and fractionation of proteoglycans using a vertical tube rotor for isopycnic centrifugation.
作者信息
Radhakrishnamurthy B, Smart F, Berenson G S
出版信息
Prep Biochem. 1980;10(2):151-9. doi: 10.1080/00327488008061730.
PMID:7383974
Abstract
A comparison was made between a vertical tube rotor and a fixed angle rotor for isopycnic centrifugation of proteoglycans. In the vertical tube rotor, isopycnic gradient was achieved much faster than in the conventional fixed angle rotor. The use of a vertical tube rotor for isopycnic centrifugation shortens the time considerably for the isolation of proteoglycans from various tissues.
摘要
对用于蛋白聚糖等密度离心的垂直管转子和固定角度转子进行了比较。在垂直管转子中，等密度梯度的形成比传统的固定角度转子快得多。使用垂直管转子进行等密度离心可显著缩短从各种组织中分离蛋白聚糖的时间。
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