[Preparation of protoplasts and localization of ribonuclease, beta-1,3-glucanase and glucosoisomerase in the fungal mycelium of Penicillium brevi-compactum].

Optimal conditions for the formation and isolation of protoplasts from the fungus Penicillium brevi-compactum were investigated. Localization of ribonuclease, glucosoisomerase and beta-1,3-glucanase in the mycelium was examined. To produce protoplasts, the mycelium was treated for 3-4 hours at 40 degrees C with the incubation mixture, containing chitinase from Actinomyces kurssanovii, lytic enzymes from Act. cellulose, lysozyme and 0.8 M mannitol as an osmotic stabilizer. The levels of activities of RNase, beta-1,3-glucanase, and glucosoisomerase were measured in the fungal mycelium before preparation of protoplasts, in the incubation mixture after their preparation, and in the protoplast lysate. The protoplast formation facilitated the release of RNase, beta-1,3-glucanase and glucosoisomerase from the fungal mycelium into the incubation mixture.