Regosz A, Zuk G
Pharmazie. 1980 Jan;35(1):24-6.
The spectrophotometric method was employed for determination of tetracycline (1) and its degradation products epitetracycline (2), anhydrotetracycline (3) and anhydroepitetracycline (4). The determination has been based on the difference in the molar absorptivities (epsilon) of 1 and 2 at 254 and 267 nm, as well as of 3 and 4 at 260 and 272 nm, respectively. The difference in epsilon at 357 and 390 nm has also been employed for the determination of 1 and 3. On the basis of these dependences, the compounds were determined both in synthetic mixtures and in some solid dosage forms.
采用分光光度法测定四环素（1）及其降解产物表四环素（2）、脱水四环素（3）和脱水表四环素（4）。该测定基于1和2在254和267nm处的摩尔吸光率（ε）差异，以及3和4在260和272nm处的摩尔吸光率差异。357和390nm处的ε差异也用于测定1和3。基于这些相关性，对合成混合物和一些固体剂型中的化合物进行了测定。
