Golovatskiĭ I D, Krasnevich A Ia, Kuzyk B D
Ukr Biokhim Zh (1978). 1980 Mar-Apr;52(2):245-7.
The paper deals with the intensity of NADPH formation in the isocytrate dehydrogenase reaction and NADPH oxidation in the lactate and malate dehydrogenase reactions. It is shown that bicarbonate, phosphate, acetate, chloride and triethanolamine buffer in concentrations of 1 . 10(-1) = 4 . 10(-1) M can inhibit these processes intensity. The inhibitory effect of the mentioned anions on the NADH-isocytrate dehydrogenase activity is considerably decreased when adding MnCl2 or MgCl2 to the incubation medium. Sucrose is established to inhibit the formation of NADPH in the isocitrate dehydrogenase reaction at 2 . 10(-1) M and higher.
本文探讨了异柠檬酸脱氢酶反应中NADPH形成的强度以及乳酸脱氢酶和苹果酸脱氢酶反应中NADPH氧化的强度。结果表明，浓度为1×10⁻¹ = 4×10⁻¹ M的碳酸氢盐、磷酸盐、醋酸盐、氯化物和三乙醇胺缓冲液可抑制这些过程的强度。当向孵育介质中添加MnCl₂或MgCl₂时，上述阴离子对NADH-异柠檬酸脱氢酶活性的抑制作用会显著降低。已确定蔗糖在浓度为2×10⁻¹ M及更高时会抑制异柠檬酸脱氢酶反应中NADPH的形成。
