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[流感病毒核衣壳某些特性的制备与研究]

[Preparation and investigation of some properties of influenza virus nucleoid].

作者信息

Zvonarev A Iu, Drynov I D, Lotte V D, Alekseeva A K

出版信息

Vopr Virusol. 1980 Mar-Apr(2):153-7.

PMID:7385786
Abstract

Influenza virus nucleoid ("core") was obtained by the treatment of virions with hydrolytic enzymes without the use of fixing agents. The "core" buoyant density in sucrose density gradient was 1.24 g/cm3. The subvirus particles are completely devoid of surface proteins, glycoproteins, and their polypeptide composition is represented by group P proteins; NP and M proteins. RNA in the core is insensitive to the effect of RNase. Morphological analysis by electron microscopy revealed the presence of spherical compact particles without spikes on their surface.

摘要

流感病毒核衣壳(“核心”)是通过用水解酶处理病毒粒子而不使用固定剂获得的。“核心”在蔗糖密度梯度中的浮力密度为1.24 g/cm³。亚病毒颗粒完全没有表面蛋白、糖蛋白,其多肽组成由P组蛋白、核蛋白(NP)和基质蛋白(M)代表。核心中的RNA对核糖核酸酶(RNase)的作用不敏感。电子显微镜形态学分析显示存在表面无刺突的球形致密颗粒。

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