Rohde W, Boschek C B, Harms E, Rott R, Scholtissek C
Arch Virol. 1979;62(4):291-302. doi: 10.1007/BF01318103.
The influenza strain 413 1,1 segregated as a stable recombinant during passage of the isolate 19/N which was obtained after double infection of chick embryo fibroblasts by virus N and the fowl plague virus (FPV) mutant ts 19. Its gene constellation was determined by molecular hybridization. Upon infection of chick embryo cells by this recombinant strain, two particle populations of high (H) and low (L) buoyant densities were produced. By biological and biochemical parameters, the H-population (delta = 1.22 g/cm3) cannot be distinguished from standard infectious influenza virus. In contrast, the noninfectious L-particles (delta = 1.14 g/cm3) lack all virus-specific glycoproteins (HA, NA) as well as the matrix protein M and are visualized by electron microscopy as spikeless particles. Significant changes in the quantitative composition of the phospholipid bilayer are evident as compared to the H-particles. In addition to the previously characterized eight genes both populations contain a variety of smaller RNA fragments which hybridize with complementary RNA and presumably represent degradation products of full-length genes.
流感毒株413 1,1在19/N分离株传代过程中作为稳定重组体分离出来，19/N分离株是鸡胚成纤维细胞被病毒N和禽瘟病毒（FPV）突变体ts 19双重感染后获得的。其基因组合通过分子杂交确定。用这种重组毒株感染鸡胚细胞后，产生了高（H）和低（L）浮力密度的两种颗粒群体。根据生物学和生化参数，H群体（δ = 1.22 g/cm³）与标准感染性流感病毒无法区分。相比之下，无感染性的L颗粒（δ = 1.14 g/cm³）缺乏所有病毒特异性糖蛋白（HA、NA）以及基质蛋白M，通过电子显微镜观察为无刺颗粒。与H颗粒相比，磷脂双层的定量组成有明显变化。除了先前鉴定的八个基因外，两个群体都含有多种较小的RNA片段，这些片段与互补RNA杂交，可能代表全长基因的降解产物。
