Quenon M, Henry P, Lejeune-Lenain C, Wolter R, Franckson J R
Ann Biol Clin (Paris). 1980;38(2):95-104.
An improved method of gas-liquid chromatography (GLC) for routine estimation of adrenal steroids in urine is presented. It was applied to the exploration of steroid output in 120 normal prepuberal children to both sexes aged 3 months-12 years. Urinary data were compared to plasmatic values of adrenal hormones estimated by radio-immuno-assays (RIA). Analysis of the relationships between steroids outputs and bone age revealed that the excretion of cortisol catabolites was expressed by a saturation curve whereas that of delta 4-3 keto androgens catabolites were expressed by geometrical pregression curves; urine dehydroepiandrosterone (DHEA) and plasma DHEA-sulfate showed outbreaks of high values beyond 6 years of age which ruled out all curve fitting attempts. The computation of these relationships allowed to determine the confidence belts of the various regressions which appear as a useful tool to estimate the delayed or accelerated character of adrenal activity in prepuberal childhood. Comparison of the various urinary (GLC) and plasmatic (RIA) parameters enabled us to comment on adrenal puberty.
本文介绍了一种改进的气-液色谱法（GLC），用于尿液中肾上腺类固醇的常规测定。该方法应用于120名3个月至12岁的正常青春期前儿童（男女均有）的类固醇分泌情况研究。将尿液数据与通过放射免疫分析法（RIA）测定的肾上腺激素血浆值进行比较。对类固醇分泌量与骨龄之间的关系分析表明，皮质醇代谢产物的排泄呈饱和曲线，而δ4-3酮雄激素代谢产物的排泄呈几何级数曲线；尿脱氢表雄酮（DHEA）和血浆硫酸脱氢表雄酮在6岁以后出现高值爆发，这使得所有曲线拟合尝试均失败。这些关系的计算可以确定各种回归的置信区间，这是评估青春期前儿童肾上腺活动延迟或加速特征的有用工具。比较各种尿液（GLC）和血浆（RIA）参数，使我们能够对肾上腺青春期进行评论。
