Smith B T, Galaugher W, Thurlbeck W M
Am Rev Respir Dis. 1980 Apr;121(4):701-7. doi: 10.1164/arrd.1980.121.4.701.
Serum samples collected from pneumonectomized rabbits 9 and 21 days after surgery significantly enhanced the incorporation of [3H]thymidine into DNA by alveolar Type II cells in vitro, but they had no effect on lung or skin fibroblasts. Activity was absent from serums collected 140 days after surgery. Active serums showed a dose-response effect at concentrations between 1% and 10% in the alveolar Type II cell culture system and the activity was heat stable. The active factor eluted from columns of Sephadex G75 at the void volume at neutral pH, but after acid-ethanol extraction and chromatography under acidic conditions, it eluted with a Kd of approximately 0.65. We propose that the active substance is a somatomedin or somatomedin-like peptide.
术后9天和21天从接受肺叶切除的兔子身上采集的血清样本，在体外能显著增强肺泡II型细胞将[3H]胸腺嘧啶核苷掺入DNA的能力，但对肺或皮肤成纤维细胞没有影响。术后140天采集的血清没有活性。在肺泡II型细胞培养系统中，活性血清在1%至10%的浓度范围内呈现剂量反应效应，且活性具有热稳定性。活性因子在中性pH条件下从Sephadex G75柱的空体积处洗脱，但经酸乙醇提取和酸性条件下的色谱分析后，以约0.65的分配系数（Kd）洗脱。我们认为活性物质是一种生长调节素或生长调节素样肽。
