Stoffel W, Grol M
Hoppe Seylers Z Physiol Chem. 1978 Dec;359(12):1777-82. doi: 10.1515/bchm2.1978.359.2.1777.
The mitochondrial enzyme 3-cis-2-trans-enoyl-CoA isomerase, responsible for the positional and geometric isomerization of beta,gamma-unsaturated fatty acyl-CoA intermediates arising during beta-oxidation of unsaturated long chain fatty acids, has been isolated from rat liver, purified to homogeneity by a heat step and a combination of gel filtration and ion-exchange chromatographic procedures. The enzyme has a molecular weight of 30,000, as determined by dodecylsulfate polyacrylamide gel electrophoresis. The isomerase has a strong tendency to form a dimer. It elutes from a calibrated Sephadex G-200 column with an apparent Mr = 60000. The basic isoelectric point, pI 9.0-9.2, is due to its high content in basic amino acids. The amino acid composition determined by the ninhydrin and o-phthalaldehyde detection method is presented.
线粒体酶3-顺式-2-反式烯酰辅酶A异构酶负责不饱和长链脂肪酸β氧化过程中产生的β,γ-不饱和脂肪酰辅酶A中间体的位置和几何异构化。该酶已从大鼠肝脏中分离出来，并通过加热步骤以及凝胶过滤和离子交换色谱法的组合纯化至同质。通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳测定，该酶的分子量为30000。该异构酶有强烈形成二聚体的倾向。它从校准的Sephadex G-200柱上洗脱时，表观分子量Mr = 60000。其碱性等电点pI为9.0 - 9.2，这归因于其碱性氨基酸的高含量。本文给出了通过茚三酮和邻苯二甲醛检测法测定的氨基酸组成。
