Wendt-Gallitelli M F, Wolburg H, Schlote W, Schwegler M, Holubarsch C, Jacob R
Basic Res Cardiol. 1980 Jan-Feb;75(1):66-72. doi: 10.1007/BF02001396.
X-ray microanalysis was used to compare chemically untreated cryosections of quick-frozen myocardial tissue in "caffeine contracture" with cryosections of normal muscle. Our goal was to find out if it is possible by means of this method to detect changes in the calcium compartmentalization of the myocardial cell occurring by changes in its functional state. While it is possible to quantitate calcium in the cisternae of sarcoplasmic reticulum of the control muscle preparation, calcium could never be detected in these compartments of caffeine-contracted muscles. In active microsomal fraction of ventricular myocardium it is possible to quantitate calcium and also to distinguish two components on account of their different ability to accumulate this element. The calcium content is different in the two components of the fraction.
采用X射线微量分析技术，对“咖啡因挛缩”状态下快速冷冻心肌组织的未经化学处理的冷冻切片与正常肌肉的冷冻切片进行化学比较。我们的目的是通过这种方法，探究是否能够检测到心肌细胞因功能状态变化而发生的钙分隔变化。虽然在对照肌肉制剂的肌浆网池内能够对钙进行定量，但在咖啡因收缩肌肉的这些区室中却从未检测到钙。在心室心肌的活性微粒体部分，可以对钙进行定量，并且由于它们积累该元素的能力不同，还可以区分出两个成分。该部分的两个成分中的钙含量不同。
