用雷尼镍脱硫后，对D-青霉胺中少量L-青霉胺进行定量测定。
Quantitative determination of small amounts of L-penicillamine in D-penicillamine after desulfurization with Raney nickel.
作者信息
Lodemann E, El-Kirdassy Z H, Wacker A
出版信息
Arzneimittelforschung. 1980;30(3):395-7.
PMID:7387748
Abstract
Commercially available D-penicillamine is desulfurized with Raney nickel at room temperature in aqueous solution. L-Valine derived from contaminating L-penicillamine is determined colorimetrically by the amino acid oxidase/peroxidase reaction, using 2,2'-azino-di-[3-ethyl-benz-thiazoline-sulfonate(6)] (ABTS). The procedure assures the quantitation of L-penicillamine contaminating D-penicillamine down to the level of 0.1%.
摘要
市售的D-青霉胺在室温下于水溶液中用阮内镍进行脱硫处理。由污染的L-青霉胺衍生而来的L-缬氨酸，通过氨基酸氧化酶/过氧化物酶反应，使用2,2'-叠氮基-二-3-乙基-苯并噻唑啉-磺酸盐(6)进行比色测定。该方法能够确保对污染D-青霉胺的L-青霉胺进行定量分析，最低检测限可达0.1%。
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