Meneghini R, Hoffmann M E
Biochim Biophys Acta. 1980 Jun 27;608(1):167-73. doi: 10.1016/0005-2787(80)90144-6.
Single-strand breaks were formed in DNA when nuclei from human fibroblasts were incubated with buffered salt solutions containing H2O2. This had not been observed when purified DNA had been exposed to H2O2. The nuclear factor which mediates the action of H2O2 on DNA was removed when nuclei were dialyzed against buffered salt solutions containing EDTA. If this complexing agent was not present during dialysis, the factor remained in the nuclear preparation. The formation of DNA single-strand breaks was considerably diminished when nuclei were incubated in solutions containing H2O2 plus hydroxyl radical scavengers. These facts favor a scheme according to which H2O2 reacts with a metal-macromolecule complex in the nucleus giving rise to a hydroxyl radical which is the DNA-damaging agent. Therefore, hydrogen peroxide seems to be a mimetic chemical of ionizing radiation.
当人成纤维细胞核与含有过氧化氢的缓冲盐溶液一起孵育时，DNA中会形成单链断裂。而当纯化的DNA暴露于过氧化氢时，并未观察到这种情况。当细胞核用含有乙二胺四乙酸（EDTA）的缓冲盐溶液透析时，介导过氧化氢对DNA作用的核因子会被去除。如果在透析过程中不存在这种络合剂，该因子会保留在核制剂中。当细胞核在含有过氧化氢和羟基自由基清除剂的溶液中孵育时，DNA单链断裂的形成会显著减少。这些事实支持了这样一种机制：过氧化氢与细胞核中的金属 - 大分子复合物反应，产生作为DNA损伤剂的羟基自由基。因此，过氧化氢似乎是一种模拟电离辐射的化学物质。
