Khasigov P Z, Kuznetsov D A, Nikolaev A Ia
Biokhimiia. 1980 Feb;45(2):378-81.
A procedure for determination of the rate of poly(A) degradation based on a comparative analysis of poly(A)-segments of impulse-labelled mRNA under conditions of subsequent blocking of poly(A) synthesis by cordycepin was developed. The method described was used to analyze the rate of shorting of the poly(A)-segment of mRNA in the liver and brain cortex of the rat during the embryonic and postnatal development. It was shown that throughout ontogenesis the rate of poly(A) degradation in liver cells is significantly higher than in brain cortex cells for both types of polyribosomal mRNAs, the rate of poly(A) degradation within free polyribosomal mRNA being higher as compared to the membrane-bound mRNA for both tissues. On the 17th embryonic day the rate of poly(A) degradation is high in both polyribosomal types; it is decreased by the moment of birth and is either increased in the postnatal life or remains unchanged (free polyribosomes of the liver and membrane-bound polyribosomes of brain cortex).
基于在虫草素对聚腺苷酸（poly(A)）合成进行后续阻断的条件下，对脉冲标记的信使核糖核酸（mRNA）的聚腺苷酸片段进行比较分析，开发了一种测定聚腺苷酸降解速率的方法。所描述的方法用于分析大鼠胚胎期和出生后发育过程中肝脏和大脑皮质中mRNA聚腺苷酸片段缩短的速率。结果表明，在整个个体发育过程中，两种类型的多核糖体mRNA在肝细胞中的聚腺苷酸降解速率均显著高于大脑皮质细胞，两种组织中游离多核糖体mRNA内的聚腺苷酸降解速率均高于膜结合mRNA。在胚胎第17天，两种多核糖体类型中的聚腺苷酸降解速率都很高；出生时该速率降低，在出生后的生命中要么增加，要么保持不变（肝脏的游离多核糖体和大脑皮质的膜结合多核糖体）。
