Rinaudo M T, Ponzetto C, Curto M, Bruno R
Ital J Biochem. 1978 May-Jun;27(3):177-90.
8 and 24 hours after alloxan administration, diabetic rat brain shows decreased glycogen content, significantly increased FDP, triose phosphates, pyruvate and lactate levels, a large rise in glucose and a 27% activation of anaerobic lactate production from glycogen. 48 hours after alloxan administration there is a recovery of glycogen and a fall in lactate levels. ATP and AMP levels are unchanged 8 and 24 hours after alloxan administration but the former is increased and the latter decreased 48 hours posttreatment. Insulin given to rats 8 hours after alloxan treatment reverses glycogen, FDP, triose phosphates, pyruvate and lactate levels seen in the diabetic rat brain. In addition the increament in glucose is reduced by half and the rate of anaerobic lactate formation from glycogen is restored to control values. G-6-P levels, unaffected by alloxan or insulin alone, are significantly lowered in animals which received insulin after alloxan. Phosphorylase, HK, PFK, ALD, GAPDH, PK, LDH and Glycogen synthetase activities are not modified in rat brain by administration of alloxan or insulin or both.
给予四氧嘧啶后8小时和24小时，糖尿病大鼠脑内糖原含量降低，1,6 - 二磷酸果糖（FDP）、磷酸丙糖、丙酮酸和乳酸水平显著升高，葡萄糖大幅增加，糖原无氧酵解生成乳酸的过程激活了27%。给予四氧嘧啶后48小时，糖原恢复，乳酸水平下降。给予四氧嘧啶后8小时和24小时，三磷酸腺苷（ATP）和一磷酸腺苷（AMP）水平未发生变化，但治疗后48小时，前者升高，后者降低。在给予四氧嘧啶8小时后给大鼠注射胰岛素，可逆转糖尿病大鼠脑内糖原、FDP、磷酸丙糖、丙酮酸和乳酸水平。此外，葡萄糖的增加量减半，糖原无氧酵解生成乳酸的速率恢复至对照值。单独使用四氧嘧啶或胰岛素对6 - 磷酸葡萄糖（G - 6 - P）水平无影响，但在给予四氧嘧啶后再注射胰岛素的动物中，G - 6 - P水平显著降低。给予四氧嘧啶、胰岛素或两者同时给药，均不会改变大鼠脑内磷酸化酶、己糖激酶（HK）、磷酸果糖激酶（PFK）、醛缩酶（ALD）、甘油醛 - 3 - 磷酸脱氢酶（GAPDH）、丙酮酸激酶（PK）、乳酸脱氢酶（LDH）和糖原合成酶的活性。
