Roubos E W, van der Wal-Divendal R M
Cell Tissue Res. 1980;207(2):267-75. doi: 10.1007/BF00237811.
The release of neuropeptides and other proteinaceous hormones by exocytosis was studied in some detail by means of tannic acid in combination with glutaraldehyde (TAGO-method). This method strongly enhances the electron density of extracellular proteins including exocytosed secretory products, and therefore facilitates visualization of the release process in qualitative and quantitative respects. This study included a variety of neurosecretory cell types in the CNS of the freshwater snail L. stagnalis, the storage and glandular lobes of the corpora cardiaca of the locust L. migratoria, and the posterior pituitary of the rat. In addition, peptide secreting cells in the anterior pituitary were examined. The cytochemical specificity of the TAGO-method as well as the significance of "frozen" contents of exocytosing secretory granules in mammotrophs and somatotrophs of the rat is discussed.
通过将单宁酸与戊二醛结合使用（TAGO法），对通过胞吐作用释放神经肽和其他蛋白质激素进行了较为详细的研究。该方法可显著增强包括胞吐分泌产物在内的细胞外蛋白质的电子密度，因此有助于从定性和定量方面观察释放过程。本研究涵盖了淡水螺静水椎实螺中枢神经系统中的多种神经分泌细胞类型、飞蝗迁徙蝗心侧体的储存叶和腺叶以及大鼠的垂体后叶。此外，还对大鼠垂体前叶中的肽分泌细胞进行了检查。讨论了TAGO法的细胞化学特异性以及大鼠乳腺营养细胞和生长激素细胞中胞吐分泌颗粒“冻结”内容物的意义。
