Buma P, Roubos E W, Buijs R M
Histochemistry. 1984;80(3):247-56. doi: 10.1007/BF00495773.
The release of neural, neuroendocrine, and endocrine secretory products by exocytosis was ultrastructurally studied by means of tissue incubation in Ringer containing tannic acid (Tannic Acid Ringer Incubation-method; TARI -method), followed by conventional fixation. Tannic acid strongly enhances the electron density of extracellular (secretory) substances. During TARI -treatment of tissues exocytosis proceeds, but the exteriorized contents of the secretory granules are immediately fixed by tannic acid and do not diffuse away into the extracellular space. In this way detection of exocytosis is markedly facilitated since the number of exocytosis phenomena visible at the ultrastructural level considerably increases with progressing incubation time. Studies of the central nervous system of the mollusc Lymnaea stagnalis show that the occurrence of exocytosis during TARI -treatment is calcium-dependent. With the TARI -method exocytosis has been clearly demonstrated in a variety of structures (endocrine cells, neurohaemal axon terminals, synapses) of L. stagnalis, the insect Locusta migratoria, and the rat, including cell types where exocytotic release had not been shown before.
通过在含鞣酸的林格氏液中进行组织孵育（鞣酸林格氏液孵育法；TARI法），随后进行常规固定，利用超微结构研究了通过胞吐作用释放神经、神经内分泌和内分泌分泌产物的情况。鞣酸能显著提高细胞外（分泌）物质的电子密度。在对组织进行TARI处理期间，胞吐作用会发生，但分泌颗粒排出的内容物会立即被鞣酸固定，不会扩散到细胞外空间。这样一来，由于随着孵育时间的延长，在超微结构水平上可见的胞吐作用现象的数量显著增加，胞吐作用的检测得到了显著促进。对软体动物椎实螺中枢神经系统的研究表明，TARI处理期间胞吐作用的发生依赖于钙。采用TARI法，已在椎实螺、昆虫飞蝗和大鼠的多种结构（内分泌细胞、神经血轴突终末、突触）中清楚地证明了胞吐作用，包括以前未显示有胞吐释放的细胞类型。
