Puurunen J
Eur J Pharmacol. 1980 May 16;63(4):275-80. doi: 10.1016/0014-2999(80)90255-1.
Gastric mucosal blood flow in ethanol-induced mucosal damage was studied in urethane-anaesthetic rats by reference to 14C-aminopyrine clearance in the gastric mucosa. Irrigation of the stomach with 30% ethanol in acid saline (100 mM HCl plus 50 mM NaCl) for 40 min broke the gastric mucosal barrier, as indicated by an increased outflow of Na+ and K+ ions and back-diffusion of H+ ions. Gastric mucosal blood flow also increased about 2-fold decreasing after cessation of ethanol irrigation along with the net ion fluxes. The increase in gastric mucosal blood flow occasioned by 10, 20 and 30% ethanol in acid saline was directly proportional to the net fluxes of H+, Na+ and K+ ions. When the stomach was irrigated with 30% ethanol in a less acid medium (10 mM HCl, 90 mM choline chloride plus 50 mM NaCl) there was still a significant increase in the outflow of Na+ and K+ ions, but only a slight-back-diffusion of H+ ions. During this low rate of acid back-diffusion 30% ethanol reduced gastric mucosal blood flow by about 50%. The results suggest that ethanol-induced mucosal damage in the rat is associated with an increase in gastric mucosal blood flow only if combined with back-diffusion of H+ ions.
通过参考胃黏膜中14C-氨基吡啶的清除率，研究了乌拉坦麻醉大鼠乙醇诱导的黏膜损伤中的胃黏膜血流量。用含30%乙醇的酸性盐水（100 mM HCl加50 mM NaCl）灌胃40分钟会破坏胃黏膜屏障，这表现为Na+和K+离子流出增加以及H+离子反向扩散。胃黏膜血流量在乙醇灌胃停止后也增加约2倍，随后随净离子通量下降。在酸性盐水中，10%、20%和30%乙醇引起的胃黏膜血流量增加与H+、Na+和K+离子的净通量成正比。当胃用酸性较弱的介质（10 mM HCl、90 mM氯化胆碱加50 mM NaCl）中的30%乙醇灌胃时，Na+和K+离子流出仍显著增加，但H+离子仅有轻微反向扩散。在这种低速率的酸反向扩散期间，30%乙醇使胃黏膜血流量减少约50%。结果表明，仅当与H+离子反向扩散相结合时，乙醇诱导的大鼠黏膜损伤才与胃黏膜血流量增加有关。
